Ripening in non-climacteric fruit like sweet cherry (Prunus avium), involves complex genetic and epigenetic changes influenced by plant hormones. Abscisic acid (ABA) plays a fundamental role in this process, particularly in regulating pigment production, fruit softening, and sugar accumulation.
In a recent study, researchers explored how ABA modulates these ripening processes at both genetic and epigenetic levels through RNA sequencing (RNA-seq) and whole-genome bisulfite sequencing (WGBS).
The RNA-seq analysis provided a comprehensive view of gene expression changes during fruit ripening in response to ABA. The study found an overrepresentation of genes associated with ABA response, secondary metabolism, and sugar synthesis. These genetic modifications support key ripening traits such as fruit color development and the accumulation of ripening markers like carotenoids and anthocyanins.
Specifically, genes related to carotene catabolism, which contribute to the rich coloration in ripe cherries, showed significant transcriptional activation. The analysis also highlighted the role of xyloglucan-modifying enzymes, associated with cell wall remodeling during ripening, suggesting that ABA influences fruit texture through these pathways.
In parallel, the WGBS analysis revealed that ABA treatment leads to DNA hypomethylation in the promoter regions of specific genes. This epigenetic change was particularly evident in genes involved in carotene catabolism and cytokinin biosynthesis. The hypomethylation status in these regions is crucial as it facilitates the transcriptional activation of these genes, further contributing to the ripening process.
The study also identified methylation variations in genes encoding members of the Aux/IAA and ARF families, known to be involved in auxin signaling pathways, indicating a broader regulatory network.
ABA's role extends beyond simple gene activation; it integrates with other hormonal pathways, creating a complex network that finely tunes the ripening process. The study found that genes encoding for enzymes involved in cytokinin biosynthesis showed altered methylation patterns after ABA treatment. Cytokinins interact with ABA to modulate various aspects of ripening. This interaction suggests a delicate balance orchestrated by ABA and cytokinin signaling, which is critical for the proper development and maturation of cherries.
The results of this study underscore the multiple roles of ABA in non-climacteric fruit ripening. ABA not only triggers significant transcriptional changes but also induces epigenetic modifications that together coordinate the complex physiological changes during ripening. Understanding these mechanisms provides valuable insights into the hormonal control of fruit
development and opens potential avenues for improving fruit quality through targeted interventions.
Source: Kuhn, N. et al. (2024). RNA-Seq and WGBS Analyses During Fruit Ripening and in Response to ABA in Sweet Cherry (Prunus avium) Reveal Genetic and Epigenetic Modulation of Auxin and Cytokinin Genes. Journal of Plant Growth Regulation, 1-23. https://doi.org/10.1007/s00344-024-11340-9.
Image: SL Fruit Service
Andrea Giovannini
University of Bologna (IT)
16 Dec 2025
Catalonia launches the release of Ganaspis kimorum, a parasitoid introduced to fight Drosophila suzukii, which causes severe damage to cherry trees. An innovative biocontrol strategy to reduce pesticide use and protect high-quality cherry production sustainably.
24 Oct 2024
An effective spray program for downy mildew and brown rot involves a combined approach of protective cover sprays and curative fungicides, as needed, from bud burst to flowering and through to harvest, depending on weather conditions and disease pressure.
23 Apr 2026
Corette® rootstocks by Agromillora show strong adaptability to Mediterranean soils in Spain. Trials highlight good yields, early production and suitability for intensive orchards, improving efficiency, fruit quality and long-term sustainability.
23 Apr 2026
A Tasmanian research project examines how rain covers alter orchard microclimates, analysing impacts on cherry quality, yield and tree growth. Findings will help growers manage hotter summers, extreme rainfall events and the increasing challenges of climate change.
