Temperature is one of the main environmental factors limiting plant growth, development and yield. One of the main abiotic stress factors that limit plant distribution, crop production and influence the suitability of locations for orchard planting is low temperature conditions.
Frosts can cause considerable economic losses and, therefore, limit the presence of orchards in areas subject to these phenomena. Differential Thermal Analysis (DTA) is a method often used to assess the cold resistance of plant organs to survive cold temperatures.
For example, flowers are mounted on thermoelectric modules and subjected to cooling temperatures to perform DTA. The water inside the biological tissue is supercooled and solidifies, releasing heat that is detected as low temperature exotherm (LTE). This is assumed to be equivalent to the temperature at which embryos suffer lethal damage.
The only critical aspect of this method is that the analyses must be carried out in the laboratory, and the timing and transport protocols can influence the values obtained. A more complete understanding of the comparison between lethal temperature measurements obtained through controlled freezing experiments with currently available instruments and those obtained with DTA is therefore needed.
The objectives of the study by the University of British Columbia and the Research and Development Centre in Summerland (Canada) were:
As evidenced by the production of low-temperature exotherms at warmer temperatures, researchers found that buds stored at warmer temperatures (12.5 °C and room temperature) for 2-6 h prior to differential thermal analysis or transported to the laboratory in a humid environment underwent biologically relevant alterations in their apparent cold sensitivity.
Through numerous tests, the researchers realised that the speed at which the temperature was lowered to achieve the low temperature exotherms also significantly influenced the results. It was seen that higher values corresponded to faster cooling. In general, the obtained low-temperature exotherms were similar between the buds, although the amount of plant material remaining attached to the base of the bud varied.
The results of this study indicate that measures of cold resilience obtained from DTA and controlled freezing experiments are generally comparable. The results of this study are relevant for researchers interested in performing cold hardiness measurements in sweet cherry. They emphasise the importance of maintaining consistency in DTA pre-test conditions and bud preparation in order to obtain reliable and comparable results between studies.
Source: Elizabeth Houghton, Kirsten Hannam, Denise Neilsen, and Louise M. Nelson. 2024. Factors that influence measurements of sweet cherry (Prunus avium) flower bud cold hardiness obtained using differential thermal analysis. Canadian Journal of Plant Science. 104(3): 204-216. https://doi.org/10.1139/cjps-2023-0087.
Image: Canadian Science Publishing
Melissa Venturi
University of Bologna (IT)
10 Mar 2026
The LTER–Sweet Cherry model uses temperature and solar radiation data to estimate floral dormancy progression, relative water content and cold hardiness. The web tool helps evaluate seasonal frost risk in sweet cherry orchards in Yakima, Washington using predictive climate data.
17 Oct 2024
During dormancy and leaf fall there are some points to consider that are very important for the productivity of the orchards. Productivity that begins with a correct entry into “Recess or Dormancy” of the plants. Stage that is key and relevant to achieve full productive potential
16 Jun 2026
Cooperation between Belarus and VNIISPK strengthens genetic improvement in apple, cherry and currant, selecting productive, disease-resistant varieties adapted to local conditions and supporting a more innovative, resilient and competitive fruit industry across Eastern Europe.
16 Jun 2026
The 2026 sweet cherry season in Italy’s Euganean Hills reflects unstable weather, strong yields and direct sales. In Vò, Laura Ambrosi explains how quality, fruit size up to 30 mm, trust and territorial identity create value for growers, tourists and consumers in Veneto.
