Temperature is one of the main environmental factors limiting plant growth, development and yield. One of the main abiotic stress factors that limit plant distribution, crop production and influence the suitability of locations for orchard planting is low temperature conditions.
Frosts can cause considerable economic losses and, therefore, limit the presence of orchards in areas subject to these phenomena. Differential Thermal Analysis (DTA) is a method often used to assess the cold resistance of plant organs to survive cold temperatures.
For example, flowers are mounted on thermoelectric modules and subjected to cooling temperatures to perform DTA. The water inside the biological tissue is supercooled and solidifies, releasing heat that is detected as low temperature exotherm (LTE). This is assumed to be equivalent to the temperature at which embryos suffer lethal damage.
The only critical aspect of this method is that the analyses must be carried out in the laboratory, and the timing and transport protocols can influence the values obtained. A more complete understanding of the comparison between lethal temperature measurements obtained through controlled freezing experiments with currently available instruments and those obtained with DTA is therefore needed.
The objectives of the study by the University of British Columbia and the Research and Development Centre in Summerland (Canada) were:
As evidenced by the production of low-temperature exotherms at warmer temperatures, researchers found that buds stored at warmer temperatures (12.5 °C and room temperature) for 2-6 h prior to differential thermal analysis or transported to the laboratory in a humid environment underwent biologically relevant alterations in their apparent cold sensitivity.
Through numerous tests, the researchers realised that the speed at which the temperature was lowered to achieve the low temperature exotherms also significantly influenced the results. It was seen that higher values corresponded to faster cooling. In general, the obtained low-temperature exotherms were similar between the buds, although the amount of plant material remaining attached to the base of the bud varied.
The results of this study indicate that measures of cold resilience obtained from DTA and controlled freezing experiments are generally comparable. The results of this study are relevant for researchers interested in performing cold hardiness measurements in sweet cherry. They emphasise the importance of maintaining consistency in DTA pre-test conditions and bud preparation in order to obtain reliable and comparable results between studies.
Source: Elizabeth Houghton, Kirsten Hannam, Denise Neilsen, and Louise M. Nelson. 2024. Factors that influence measurements of sweet cherry (Prunus avium) flower bud cold hardiness obtained using differential thermal analysis. Canadian Journal of Plant Science. 104(3): 204-216. https://doi.org/10.1139/cjps-2023-0087.
Image: Canadian Science Publishing
Melissa Venturi
University of Bologna (IT)
24 Dec 2024
Developing a fruit-growing project in southern Chile is different from what happens in other production areas. Here, a warm humid temperate climate and volcanic soils dominate, which result in late production and risks of climatic events during critical phenological periods.
23 May 2024
‘The objective of this new version is to offer the market a packaging machine with greater capacity and gentler handling of the fruit,’ says Pedro Martínez, sales manager, ‘in order to consolidate our position in the European market with a wider range of products'.
18 Sep 2025
A recent study in Poland confirms the effectiveness of 1-MCP in extending sweet cherry shelf life. The treatment reduces weight loss and preserves visual and nutritional quality. Promising results for Kordia and Regina varieties after 21 days in cold storage.
18 Sep 2025
New research shows that cherries, rich in antioxidants and anti-inflammatory compounds, can lower the risk of chronic diseases like diabetes, stroke and heart disease. Including them daily supports cardiovascular health, blood sugar regulation and overall wellbeing.
