The progress in sweet cherry (Prunus avium L.) breeding has been marked by the identification of molecular markers for crucial traits, utilizing genome sequencing for marker-assisted selection (MAS). Despite these achievements, quantitative trait locus (QTL) analyses have often lacked resolution, leading to missed gene associations.
Traditional breeding methods face challenges related to low efficiency and high costs. Recent developments in whole-genome sequencing have uncovered nearly 2 million single nucleotide polymorphisms (SNPs), promising higher marker density for genome-wide association studies (GWAS). However, uneven SNP distribution remains an obstacle, hindering the identification of candidate genes. The current hurdle is to leverage GWAS potential for high-resolution mapping to expedite the breeding of improved sweet cherry varieties.
Researchers employed paired-end sequencing on the Illumina NovaSeq 6000 platform, generating a 4.15-Tb sequence with samples contributing 7.6–36.8 Gb each. The extensive genome coverage allowed the identification of 1,767,106 high-quality SNPs distributed across the accessions. Principal component analysis (PCA) and Bayesian information criterion (BIC) indicated a homogeneous population without distinct genetic subgroups.
Phenotypic diversity assessed from 2012 to 2020 revealed significant variability across traits, with Collection of Genetic Resources (CGR) accessions displaying more pronounced diversity than breeding materials (BM). Notable differences in color traits and fruit size were observed, aligning with the goals of selective breeding.
Collection of Genetic Resources population structure analysis. A) Principal component (PC) analysis plot of the first two PCs identified from 235 accessions based on 1,767,106 single nucleotide polymorphisms. B) Heat map of a kinship matrix estimated using the VanRaden algorithm. Both plots were generated with GAPIT.
Source: EurekAlert!
Image: High-resolution genome-wide association study of a large Czech collection of sweet cherry (Prunus avium L.) on fruit maturity and quality traits - Scientific Figure on ResearchGate. https://www.researchgate.net/figure/Collection-of-Genetic-Resources-population-structure-analysis-A-Principal-component_fig1_364394570.
13 Aug 2025
In southern Chile, reflective ground films in high-density cherry orchards under plastic tunnels improve the quality of Regina cherries by enhancing firmness, sugar levels, and color. Proper installation timing is essential to reduce cracking and postharvest defects.
16 Aug 2024
According to the results of a Canadian study the sweet cherry lethal temperature models devised for 'Sweetheart' and 'Lapins' showed potential when applied to other cultivars with comparable flowering duration, such as 'Sonata', 'Staccato' and 'Skeena'.
08 Jan 2026
Essential post-harvest practices to increase cherry orchard yield in Chile. Learn how irrigation, nutrition and pest control help optimize plant recovery, build reserves, and ensure a productive flowering and fruiting season under Chile’s changing climate conditions.
08 Jan 2026
A genomic study identified 235 UGT genes in sweet cherry, revealing the key role of PavUGT10 in waterlogging tolerance. The gene boosts plant survival by enhancing antioxidant responses and reducing oxidative stress damage in flooded conditions.
